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Prolactin does have a significant acute increase after ejaculation. It does, to some degree, after orgasm as well. But prolactin acts on the pituitary to inhibit the release of the hormones LH and FSH, of which LH can increase
testosterone.
Kyle Gillett
Frequent Masturbation Increases Prolactin, Inhibiting LH Release & Lowering Libido
Dr. Kyle Gillett: Tools for Hormone Optimization in Males
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